Day7
3.0 High-Efficiency NK Serum-Free Culture Kit | Chapter 3: CBNK Competitor Comparison
Release Date:
2026-04-10 11:57
Hycells Biology 3.0 High-Efficiency Version of HiXpan Registered trademark The NK serum-free culture kit is manufactured in strict compliance with GMP standards, and its production system adheres to relevant guidelines, including the “818 Requirements,” making it suitable for pharmaceutical-grade manufacturing and applications.
In response to widespread customer demand, we have conducted a comparative analysis of culture performance against two leading commercial reference media.
Competitor Benchmark Data Sharing
Comparison of Competing Products 1 and 2 with Hycells 3.0 High-Efficiency HiXpan Registered trademark Amplification performance of the NK serum-free culture kit on cryopreserved CBMCs:
Using the same cryopreserved CBMCs as seed cells, inoculate at an initial density of 5 × 10^7 cells, and separately employ Competitor Product 1, Competitor Product 2, and the 3.0 High-Efficiency Version of HiXpan. Registered trademark Three serum-free NK cell culture kits were used to perform continuous expansion, with the 2 L culture medium being completely consumed over a total cultivation period of 16 days.
Perform cell counts every 2–3 days and assess cell purity on days 7, 10, and 16.
On day 16, harvest the expanded cells and assess their cytotoxic activity.
Cell Expansion Status (Donor 1)




On Day 16, expanded NK cells were harvested and used to kill K562-Luc cells.

Raw Flow Cytometry Plot
Day 7 Day 10 Day 16
Cell Expansion Status (Donor 2)




On Day 16, expanded NK cells were harvested and used to kill K562-Luc cells.

Raw Flow Cytometry Plot
Related News
(II) Security Assurance Section | Safety First, Defense Prioritized
Compliance‑driven traceability establishes a clear “identity” for the product, but the real test lies in safety. While a multi‑donor pooling strategy ensures batch‑to‑batch consistency, it also inevitably elevates the risk of pathogen introduction. In the face of these associated risks and challenges, Heyousheng has never compromised on its manufacturing standards—relying on more precise design to deliver on its promise of enhanced safety.
This article will examine how He Yousheng proactively integrates the quality management system into the hospital setting, translating compliance requirements into concrete, actionable practices starting at the sample collection stage, thereby establishing a trust‑based chain that can withstand audit scrutiny.
In selecting hPL raw materials, Heyousheng consistently upholds the principle that “safety is determined at the source.” The samples used in production are rigorously collected in accordance with applicable standards and undergo stringent donor screening, effectively mitigating the risk of disease transmission at the very outset. Moreover, from informed consent forms and ethical approvals to clinical‑grade collection under the GCP framework, Heyousheng has established a fully traceable documentation chain, ensuring that every sample is accounted for and verifiable. In contrast to the industry’s longstanding reliance on the conventional model of using expired blood supplies from blood banks, this approach represents a structural, generational upgrade.
Recently, the research team led by Researcher Bian Yingjie at the Guangzhou National Laboratory, in collaboration with partners from Sun Yat-sen University, published a study titled “Iron overload in the tumor microenvironment induces CD8⁺ T cell ferroptosis and dysfunction” in the internationally renowned academic journal Nature Communications.
Hycells Recruitment | Never Stop Exploring, Never Stop Innovating
The company consistently upholds its core values of “people‑centricity, quality first, and innovation as the foundation,” remains driven by technological innovation, and sets new industry benchmarks for domestic innovation, thereby laying a solid foundation for the development and global advancement of China’s innovative pharmaceutical sector.
The HiXpan® NK Serum-Free Culture Kit, Version 3.0, is manufactured in strict compliance with GMP standards, and its production system fully adheres to relevant guidelines and regulations, including the “818 Requirements,” positioning it for pharmaceutical‑grade manufacturing and applications.





