3.0 High-Efficiency NK Serum-Free Culture Kit | Chapter 3: CBNK Competitor Comparison


Hycells Biology 3.0 High-Efficiency Version of HiXpan Registered trademark The NK serum-free culture kit is manufactured in strict compliance with GMP standards, and its production system adheres to relevant guidelines, including the “818 Requirements,” making it suitable for pharmaceutical-grade manufacturing and applications. 
 

In response to widespread customer demand, we have conducted a comparative analysis of culture performance against two leading commercial reference media. 
 

Competitor Benchmark Data Sharing

Comparison of Competing Products 1 and 2 with Hycells 3.0 High-Efficiency HiXpan Registered trademark Amplification performance of the NK serum-free culture kit on cryopreserved CBMCs:

Using the same cryopreserved CBMCs as seed cells, inoculate at an initial density of 5 × 10^7 cells, and separately employ Competitor Product 1, Competitor Product 2, and the 3.0 High-Efficiency Version of HiXpan. Registered trademark Three serum-free NK cell culture kits were used to perform continuous expansion, with the 2 L culture medium being completely consumed over a total cultivation period of 16 days.

Perform cell counts every 2–3 days and assess cell purity on days 7, 10, and 16.

On day 16, harvest the expanded cells and assess their cytotoxic activity.

01
 

Cell Expansion Status (Donor 1)

 


 

 


 

On Day 16, expanded NK cells were harvested and used to kill K562-Luc cells. 
 


 


 


 


 


 

Raw Flow Cytometry Plot

 

Day 7 Day 10 Day 16 
 

02
 

Cell Expansion Status (Donor 2)


 


 


 

On Day 16, expanded NK cells were harvested and used to kill K562-Luc cells. 
 

Day7

Day 10

Day16


 


 


 


 

Raw Flow Cytometry Plot

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