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3.0 High-Efficiency Version of HiXpan® NK Serum-Free Culture Kit | Chapter 4: Comprehensive Overview of Customer-Specific Culture Data Across Multiple Scenarios

Release Date:

2026-07-31 11:28

Hycells 3.0 High-Efficiency Version HiXpan ® The NK serum-free culture kit is manufactured in strict compliance with GMP standards, and its production system fully meets relevant guidelines and regulations, including the “818 Requirements,” positioning it for pharmaceutical‑grade manufacturing and application scenarios. In response to customers’ demand for multi‑source, multi‑scenario culture data, our company has systematically compiled data from more than 20 clients, covering three major application types: PBMC‑derived, CBMC‑derived, and Cell Feeder–based bottom‑permeable flask cultures. The data demonstrate that HiXpan® NK… Serum-free culture The kit demonstrates excellent batch-to-batch consistency and process robustness in practical applications, while also providing guidance for further process optimization:

  • Effective maintenance and enhancement of purity in PBMC cultures;
  • Develop refined control over critical process steps, such as bag transfer and bag sorting.
  • Systematic promotion and application of high-density cultivation modes.

We will continue to drive product upgrades with data, helping NK cell culture processes advance toward greater efficiency, stability, and scalability.

 

Data sharing and summarization for multi-scenario customer development


 

01
 

CBMC Training Data Visualization


 

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Beijing Customer 2026.03.12

Hunan customer 2026.03.20

Yunnan Customer 2026.03.28

Jiangsu Customer 2026.04.19

Jilin Customer 2026.04.22

Nanjing Customer 2026.05.09

Hunan customer 2026.05.11

Beijing customer 2026.05.11

Shenzhen Customer 2026.05.24

Nanjing Customer 2026.06.05

Chongqing Customer 2026.06.08

Yantai Customer 2026.06.22

Yantai Customer 2026.06.28

Shenzhen Customer 2026.07.01

Jinan Customer 2026.07.01

In terms of amplification efficiency, the highest‑yield batch D18 achieved a 714‑fold amplification, with a final viable cell count of 4.0 × 10. 10 The final purity reaches 99%, with a culture volume of 12,000 mL; the lowest‑performing batch, D12, achieves a viable cell count of 4.5 × 10⁹. Batch‑to‑batch differences in expansion efficiency can be as high as ninefold. Extending the culture duration and scaling up the bioreactor system can help mitigate these variations, allowing customers to make flexible choices based on their specific process requirements.

Process analysis indicates that the bag‑transfer step at D7 and the bag‑splitting operations from D9 to D11 are critical process steps that influence the efficiency of the final amplification. D15 is the most common harvest time point, while D16–D18 represent a high‑yield window.

02
 

PBMC Culture Data Presentation


 

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Hebei Customer 2026.03.14

Shanghai Customer 2026.04.24

Xinjiang customer 2026.05.06

Shenzhen Customer 2026.06.01

Shanghai Customer 2026.06.02

Shandong customer 2026.07.05

Six batches of PBMC-derived culture data show that the final viable cell counts ranged from 2.4 × 10⁹ to 1.85 × 10 10 , cultivation period D13–D19, initial inoculum size 0.39×10⁸–0.68×10⁸.

 

03
 

Cell Feeder Bottom-permeable bottle culture Summary


 

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Xi’an Customer 2026.01.08

Hangzhou Customer 2026.04.01

Hainan Customer 2026.06.01

Beijing customer 2026.07.05

Wuhan Customer 2026.07.06

Jilin Customer 2026.07.06

The advantages of high-density expansion inCell Feeder（ Cell Culture Flask with Transparent Bottom） culture have been consistently validated across all six collaborating institutions, demonstrating excellent process scalability.

Based on the above cultivation data, the following process principles can be summarized:

The recovery of viability is highly correlated with the amplification outcome. Taking a customer in Shenzhen as an example, during the culture process, viability improved from 88% on Day 7 to 98% by Day 9, accompanied by a final amplification factor of 714‑fold, indicating that the dynamic recovery of viability is more informative for process optimization than the absolute initial viability value.

The high-density advantage of bottom‑permeable flask cultivation was consistently reproduced across six independent batches, confirming that this culture mode is… NK cell expansion It possesses clear process‑scale‑up potential and is well suited for applications that demand high cell productivity per unit volume.

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3.0 High-Efficiency Version of HiXpan® NK Serum-Free Culture Kit | Chapter 4: Comprehensive Overview of Customer-Specific Culture Data Across Multiple Scenarios

The HiXpan® NK Serum-Free Culture Kit, Version 3.0, is manufactured in strict compliance with GMP standards, and its production system fully adheres to relevant guidelines and regulations, including the “818 Requirements,” positioning it for pharmaceutical‑grade manufacturing and applications.