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3.0 High-Efficiency NK Serum-Free Culture Kit | Chapter 2: PBNK Derived from Peripheral Blood

Release Date:

2026-04-01 18:00

HeYouSheng Biotechnology’s newly launched 3.0 High-Efficiency Serum-Free NK Cell Culture Kit (Catalog No.: NK888-2L), in collaboration with the PBMC Seed Bank, provides a comprehensive, integrated cell culture solution: from thawing and recovering PBMC seed stocks or directly and efficiently expanding fresh PBMCs, to ultimately obtaining NK cells with high purity and high viability.

 

He Yousheng Biology 3.0 High-Efficiency Version of HiXpan Registered trademark The NK serum-free culture kit is manufactured in strict compliance with Good Manufacturing Practice (GMP) and its production system meets relevant guidelines, including the “818 Requirements,” making it suitable for pharmaceutical-grade production and applications.

 

Sharing of NK Cell Culture Data Derived from Peripheral Blood

Sample source: Four random samples of fresh or cryopreserved PBMCs derived from peripheral blood were obtained from the HeyouSheng Biological Cell Bank.

NK kit catalog number: NK888-2L

Initial cell count: 40 million

Cell yield: 6.8 billion–8.3 billion

Training System: 2L

Harvest Purity: 90%-94%

Amplification fold: 170–260 times

 

The fluid replacement procedure is shown in the figure below:

01
01 

Fresh PBMC


 
 

Amplification factor

Purity Data

 
 


 


 


 

Raw Flow Cytometry Plot

Day 7 Day 11 Day 14

02
 

Cryopreservation of PBMC


 

Amplification factor

Purity Data


 


 


 


 

Raw Flow Cytometry Plot

Day 7

Day 11

Day 14

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3.0 High-Efficiency NK Serum-Free Culture Kit | Chapter 3: CBNK Competitor Comparison

First Release | Off-the-Shelf PBMCs Compliant with GMP and Eligible for Dual FDA–CFDA Submission Enable Compliance-Oriented iPSC Reprogramming Starting from “Zero”

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