Amplification factor
3.0 High-Efficiency NK Serum-Free Culture Kit | Chapter 2: PBNK Derived from Peripheral Blood
Release Date:
2026-04-01 18:00
HeYouSheng Biotechnology’s newly launched 3.0 High-Efficiency Serum-Free NK Cell Culture Kit (Catalog No.: NK888-2L), in collaboration with the PBMC Seed Bank, provides a comprehensive, integrated cell culture solution: from thawing and recovering PBMC seed stocks or directly and efficiently expanding fresh PBMCs, to ultimately obtaining NK cells with high purity and high viability.
He Yousheng Biology 3.0 High-Efficiency Version of HiXpan Registered trademark The NK serum-free culture kit is manufactured in strict compliance with Good Manufacturing Practice (GMP) and its production system meets relevant guidelines, including the “818 Requirements,” making it suitable for pharmaceutical-grade production and applications.
Sharing of NK Cell Culture Data Derived from Peripheral Blood
Sample source: Four random samples of fresh or cryopreserved PBMCs derived from peripheral blood were obtained from the HeyouSheng Biological Cell Bank.
NK kit catalog number: NK888-2L
Initial cell count: 40 million
Cell yield: 6.8 billion–8.3 billion
Training System: 2L
Harvest Purity: 90%-94%
Amplification fold: 170–260 times
The fluid replacement procedure is shown in the figure below:
Fresh PBMC
Purity Data
Raw Flow Cytometry Plot
Day 7 Day 11 Day 14
Cryopreservation of PBMC
Amplification factor
Purity Data
Raw Flow Cytometry Plot
Day 7
Day 11
Day 14
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