Hycells New Product | Ultra-High-Density MSC Serum-Free Culture Medium
Release Date:
2024-11-20 14:42
Background Introduction
Mesenchymal stem cells (MSCs) are multipotent stem cells that possess all the hallmark properties of stem cells: self-renewal and the capacity for multi-lineage differentiation. MSCs were first identified in bone marrow, and subsequent studies have revealed their presence in numerous tissues throughout human development. Currently, MSCs can be isolated and cultured from a variety of sources, including bone marrow, adipose tissue, synovial membrane, bone, muscle, lung, liver, pancreas, as well as amniotic fluid and umbilical cord blood; among these, bone-marrow–derived MSCs are the most widely used.
Mesenchymal stem cells possess multi-lineage differentiation potential, the ability to support hematopoiesis and promote engraftment of hematopoietic stem cells, immunomodulatory functions, and ease of isolation and culture, among other advantageous characteristics, which have increasingly drawn attention. With the maturation of related technologies, clinical studies are now underway in many countries. Clinically, these cells are primarily used as seed cells to treat a variety of refractory diseases characterized by tissue, cellular, and organ damage that the body cannot repair spontaneously; they also serve as immunomodulatory cells for the treatment of immune rejection and autoimmune disorders.
The MSC Serum-Free Culture Kit (Catalog No.: MSC330-500 mL) is a high-quality, complete culture system specifically designed for the primary isolation and serial passaging of human mesenchymal stem cells under serum-free, xeno-free conditions. This kit supports primary isolation and culture of MSCs using methods such as explant culture and enzymatic digestion, while maintaining genomic stability over prolonged culture periods and multiple passages. It also effectively preserves key cellular characteristics and sustains balanced lineage differentiation potential, including the ability to differentiate into osteoblasts, chondrocytes, and adipocytes.
Product Advantages
Serum-free and free of xenogenic animal-derived components
Applicable to Multiple tissue sources human mesenchymal stem cells, such as umbilical cord MSCs, bone marrow MSCs, and adipose tissue MSCs
Faster proliferation capability, stability Passaged to more than 20 generations , to better maintain cellular differentiation potential
Use directly, No additional components are required. ， No coating of the culture plate is required.
The cells are small in size, and 20 million cells can be harvested within 72 hours of T175 culture.
MSC Serum-Free Culture Kit Verification Data Display
Product Performance Data Verification
1. Morphological images of umbilical cord MSCs cultured using the MSC serum-free culture kit
Morphological Characteristics of Umbilical Cord MSCs
2. HiXpan ® Under MSC culture conditions, umbilical cord MSCs exhibit a shorter doubling time and yield a higher cell harvest after three consecutive passages.
Doubling Time of Umbilical Cord MSCs Across Different Brands
Umbilical Cord MSC Yield by Brand
3. HiXpan ® Under MSC culture conditions, umbilical cord MSCs can maintain their cellular characteristics very well. Maintaining balanced lineage differentiation potential
The positive rates for flow cytometric detection of the cell surface markers CD105, CD73, and CD90 were all in Over 98% ；
The positive rates of cell surface negative markers CD45, CD34, CD19, and HLA-DR are in Less than 1% 。
4. Exosome concentration of umbilical cord mesenchymal stem cells (MSCs) (adherent culture)
The concentration of exosomes derived from umbilical cord mesenchymal stem cell (MSC) culture can reach 5E10 particles/mL.
Competitor Comparison and Validation
1.6 × 10^6 cells were seeded in a T175 flask at 20 mL (P4 harvest at 71 hours).
2.5 × 10^6 cells were seeded into a T175 flask at 30 mL (P4 harvest at 71 hours).
Compared with competitors, HiXpan ® Under MSC culture conditions, MSCs exhibit a shorter doubling time. Achieve a higher amplification fold 。
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