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Technical Service Platform

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In vitro–differentiated Th1/Th17/Treg cells

CD4+ helper T cells (Th cells) serve as mediators of cellular immunity and play a critical role in activating other immune cells, such as B cells and cytotoxic T cells, as well as in regulating immune responses.

Antibody-Dependent Cell-Mediated Cytotoxicity Assay (ADCC)

Antibodies, as integral components of the immune system, play a crucial role in defending against disease. Antibody-dependent cell-mediated cytotoxicity (ADCC) is one of the mechanisms by which antibodies exert their effector functions: when IgG antibodies specifically bind via their Fab fragments to antigenic epitopes on the surface of target cells—such as virus-infected cells and tumor cells—the Fc portion of the antibody can engage Fc receptors on effector cells, including natural killer (NK) cells, monocytes–macrophages, and neutrophils, thereby triggering the effector cells’ cytotoxic activity and directly killing the target cells. The ability to elicit ADCC against target cells is an important functional criterion for antibody candidates that are directed against cancer-associated antigens.

Antibody-dependent cellular phagocytosis

Antibody-dependent cellular cytotoxicity (ADCC) is one of the mechanisms by which antibody-based therapies exert their antitumor and other therapeutic effects. Currently, therapeutic strategies aimed at enhancing macrophage responses to therapeutic antibodies have garnered significant attention from researchers, including the identification of novel targets and the development of antibodies with enhanced functionality.

Complement-dependent cytotoxicity (CDC)

Complement is a group of heat-labile, enzymatically active proteins found in human and vertebrate serum and tissue fluids, comprising more than 30 soluble and membrane-bound proteins. Complement-dependent cytotoxicity (CDC) refers to the lytic effect on target cells resulting from the formation of a membrane attack complex after complement is activated by specific antibodies that bind to corresponding antigens on the cell membrane via the classical pathway of complement activation. Initially, antibodies bind to complement component C1q, which then triggers the sequential activation of C2 through C9 to form the membrane attack complex, ultimately leading to lysis of the target cell.

Cytokine Release Syndrome Risk Assessment (CRS)

Cytokine release syndrome (CRS) refers to a hyperactive immune response that occurs following infection with pathogenic microorganisms, leading to the rapid activation of numerous immune cells and the massive release of multiple cytokines—including TNF-α, IL-1, IL-6, IL-12, IFN-α, IFN-β, and IFN-γ—within a short period. This results in a severe systemic inflammatory response syndrome. The excessive production of these cytokines can damage tissues and organs, thereby giving rise to a wide range of clinical manifestations. Currently, the standard approach is to closely monitor and target the specific cytokines that trigger the cytokine storm.

Flow Cytometry-Based Cell Characterization Experiments (FACS)

The targets of antibody drugs are primarily disease-associated antigens or specific receptor molecules on the cell surface. Competitive binding between ligands and antibodies is assessed by using flow cytometry to determine the population of antigen-positive cells. By employing antigen-presenting cells in these assays, the spatial conformation of surface antigens more closely resembles their in vivo configuration, thereby yielding results that better reflect physiological conditions.

Antibody-dependent cellular phagocytosis

ADCP is one of the mechanisms by which antibody drugs exert their therapeutic effects in tumors and other diseases. Currently, therapeutic strategies aimed at enhancing macrophage responses to therapeutic antibodies have garnered significant attention from researchers, including Exploring new targets and developing antibodies with enhanced functionality Wait.

Antibody-based therapies have been proven to be an effective approach for treating diseases. By engaging Fc receptors (FcγRs) on the surface of immune cells via their Fc region, antibodies can elicit potent cytotoxic activity in certain effector cells; in addition to antibody-dependent cell-mediated cytotoxicity (ADCC), this includes antibody-dependent cellular phagocytosis (ADCP). ADCP is a mechanism in which an antibody simultaneously binds both to target cells—such as tumor cells—and to effector cells, such as macrophages, thereby triggering the effector cell to phagocytose the target cell. Following phagocytosis, acidification within the phagosome leads to the digestion and degradation of the target cell inside the effector cell. ADCP is considered the primary mechanism of action for several biologic agents, including those targeting CD20, CD38, EGFR, and HER-2. Meanwhile, macrophage-mediated phagocytosis of tumor cells—achieved by using anti-CD47 antibodies to block the anti-phagocytic CD47–SIRPα interaction—has shown promising results in preclinical xenograft models of various human malignancies.

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Service Advantages:

Quality System

1. Strictly adhere to the protocols for subject screening, sample collection, and sample management.

2. Complete ethical approval documentation that complies with the review requirements of the ethics committee and other regulatory compliance obligations, including corporate audits, and supports on-site inspections by clients.

3. A professional cold-chain logistics collaboration system ensures compliant and efficient delivery of samples throughout the entire process.

R&D System

1. Focused on cell therapy, gene therapy, and the development of large-molecule drugs.

2. Continuously optimize standardized preparation processes and lead industry service standards.

3. Innovate service models and expand the boundaries of research applications

Professional Team

1. A seasoned R&D team and a mature, standardized cell preparation process and manufacturing system.

2. Standardized donor registry and management system covering both Chinese and international populations

Core Resources

1. World-class production and service equipment, state-of-the-art laboratory facilities, and a nearly 1,000-square-meter R&D center.

2. Establish multiple clinical collaboration centers, with the East China region as the leading hub, covering tertiary hospitals nationwide and high-quality GCP management centers.

3. Our in-house specialized project team can provide clients with services such as regulatory interpretation, policy analysis, domestic-investor qualification determination, and approval for foreign-invested cooperation, offering one-stop compliance support.

Keyword

Antibody-Dependent Cell-Mediated Cytotoxicity Assay (ADCC)

Complement-dependent cytotoxicity (CDC)

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Antibody-Dependent Cell-Mediated Cytotoxicity Assay (ADCC)

Complement-dependent cytotoxicity (CDC)