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HiXpan ® Serum-Free γδ T Cell Culture Kit
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Introduction to γδ T Cells
γδ T cells represent an emerging frontier in tumor immunology; like αβ T cells, they can exert cytotoxic effector functions (ADCC) and produce pro-inflammatory cytokines. The key distinction is that γδ T cells recognize tumor antigens in an MHC-unrestricted manner, endowing them with the characteristics of a universal cell therapy product. The TCRγδ on the surface of γδ T cells does not require binding to MHC molecules or antigen presentation by antigen-presenting cells (APCs); instead, it can directly recognize and bind antigenic molecules. However, the repertoire of TCRγδ variants is limited and lacks diversity, resulting in a relatively narrow antigen-recognition spectrum for γδ T cells. γδ T cells in different tissues express distinct TCRγδ receptors and recognize antigens with varying properties, whereas γδ T cells within the same tissue typically express the same TCRγδ and recognize antigens of the same type.
To date, no serious adverse reactions associated with γδ T-cell therapy have been reported. This therapeutic approach has demonstrated robust efficacy in both hematologic malignancies and solid tumors, independent of specific MHC expression. By sensing stress signals uniquely expressed by cancer cells while sparing healthy cells, γδ T-cell therapy exhibits minimal toxicity and virtually no side effects, thereby holding great promise for broader clinical applications.
The use of clinically established, highly efficient, and safe in vitro expansion methods to generate standard γδ T cells has become an essential step in the treatment of malignant tumors; consequently, the development of an effective and safe in vitro γδ T-cell expansion system represents a pressing challenge that urgently needs to be addressed.
Provided by Heyousheng Biotechnology
γδ T Cell Samples & Expansion Kit
Heyou Sheng Bio offers γδ T-cell donor pre-screening services, in conjunction with the company’s proprietary HiXpan product. Registered trademark The γδT cell expansion kit is used to activate and proliferate γδT cells.
HiXpan Registered trademark γδ T Cell Expansion Kit (1 L)
HiXpan ® γδ T Cell Expansion Kit
for the culture and expansion of human γδT cells
Robust expansion of γδT cells with high yield and frequency
Feeder- and serum-free culture conditions
Expanded γδ T cells are functional with cytotoxic potential.
HiXpan ® γδ T Cell Expansion Kit [Operating Procedure]
HiXpan Registered trademark γδ T Cell Expansion Kit for the culture and expansion of human γδT cells
Single-donor blood may be used as the starting sample;
Alternatively, cryopreserved PBMCs may be used as the starting sample;
On days 3–5, retroviral transduction is recommended.
Maintain the optimal cell density during the expansion phase at 1 × 10 6 cells/mL;
Optimal γδ T cells with superior phenotypic and functional characteristics can be harvested on days D18–D22.
The infusion dose range is 1–20 × 10. 7 cells/kg.
HiXpan ® γδ T Cell Expansion Case
Three healthy donors (H01, H02, H03): for the culture and expansion of human γδT cells
Frozen PBMCs were used as the starting material;
Amplified 250-fold;
D14 purity > 90%;
Statistics: Proportion Distribution of γδ T Cells from Healthy Donors (15 Cases)
Initial γδ T cell ratio
Proportion of activated γδ T cells (Day 7)
Note: The initial positive rate of γδ T cells is not correlated with the proportion of expanded γδ T cells; however, a higher initial proportion of γδ T cells is associated with a higher success rate of expansion.
HiXpan ® Phenotypic Analysis of γδ T Cells (Day 10)
Note: γδ T cells exhibit high expression of molecules such as HLA-DR, CD56, and NKG2D, suggesting that they may possess NK-like antitumor cytotoxic activity.
HiXpan Registered trademark gamma delta T Cell Phenotype Analysis
γδ T phenotype: for the culture and expansion of human γδT cells
High expression of CD56;
High expression of NKG2D;
High expression of HLA-DR;
Express CD28;
Expression of chemokine receptors CXCR3/4 and CCR2;
Does not express PD1/CD137L/CD27/CXCR5;
Hint: γδ T cells express CXCR3, CXCR4, CCR2 The presence of chemokine receptors suggests that γδ T cells may possess unique chemotactic properties.
For more details on HeYouSheng Bio’s γδ T-cell expansion and culture media, please contact our sales representative. Alternatively, you can scan the QR code below to request a free sample of the media. Limited quantities—first come, first served.
Contact: Mr. Xie, 15201775322.
Download related documents for the γδ T-cell expansion kit (cytokine-based method):
γδ T Cell Serum-Free Culture Kit (Cytokine-Based) 2 L – Instruction Manual – 20250909
γδ T Cell Serum-Free Culture Kit (Cytokine-Based) 1 L – Instruction Manual – 20250909
Related document downloads:
Serum-Free γδ T Cell Culture Kit (Cytokine-Based) 2 L – Instruction Manual
γδ T Cell Serum-Free Culture Kit (Cytokine-Based) 1 L – Instruction Manual
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