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Sample Service

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Sample Service

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Leukopak Collection Service

Leukopak is a concentrated blood fraction collected using a apheresis machine, primarily intended for the isolation of white blood cells (WBCs) from peripheral blood. WBCs are highly enriched in peripheral blood mononuclear cells (PBMCs), which consist mainly of T cells, B cells, NK cells, and monocytes. The WBC concentration in Leukopak is substantially higher—approximately 4–7 × 10^8 WBCs per milliliter—than that in whole blood, where the average is 5–7 × 10^6 WBCs per milliliter. In addition, the red blood cell (RBC) count in Leukopak is markedly reduced, with a ratio of about 2 RBCs to 1 WBC, whereas in whole blood the ratio is roughly 1,000 RBCs to 1 WBC. Moreover, the purity and quality of the WBC fraction in Leukopak are far superior to those in whole blood, typically containing more than 20-fold the amount of PBMCs. PBMCs are highly valued for their applications in immunotherapy research, cell therapy process development, and clinical settings. Consequently, WBCs have become the preferred starting material for cell therapy research and drug development.

PBMC Isolation Service

PBMC (peripheral blood mononuclear cell) refers to mononuclear cells in peripheral blood, including lymphocytes, monocytes, dendritic cells, and a small number of other cell types such as hematopoietic stem cells. Based on the source of the sample, PBMC can be classified as whole-blood–derived PBMC or apheresis–derived PBMC. Apheresis–derived PBMC is a component blood product obtained by leukapheresis using an apheresis machine; the majority of cells in this fraction are PBMC, with only a small proportion of granulocytes and erythrocytes.

CD3+ Cell Sorting Service

CD3 is an important leukocyte differentiation antigen that is expressed on the surface of nearly all T cells. It consists of four genetic loci and six polypeptide chains, and serves as a membrane-associated antigen that forms part of the T-cell antigen receptor (TCR). Upon antigen–TCR engagement, CD3 mediates intracellular signal transduction, regulates T-cell differentiation—including the lineage commitment and activation of helper T cells and cytotoxic T cells—facilitates cytokine secretion and apoptosis, and influences ligand-receptor interactions, thereby playing a critical role in the diverse functional activities of T cells.

CD4+ Cell Sorting Service

CD4 cells refer to T lymphocytes that express the CD4 molecule on their surface; they are a crucial type of immune cell in the human body. Because HIV primarily targets CD4+ T cells, the results of CD4 cell count testing play an important role in assessing the efficacy of AIDS treatment and evaluating the patient’s immune function.

CD4+ Naive T Cell Sorting Service

Among helper T cells, naïve CD4+ T cells that have not yet been stimulated by antigens are designated as Th0. Upon maturation and activation, these cells can execute a variety of antigen-dependent effector functions. Based on their distinct functional profiles, CD4+ T cells can be classified into several Th cell subsets, including Th1, Th2, Th3, Treg, Tr1, Tfh, Th17, Th9, and Th22. These Th subsets engage in intricate interplay and coordinate their activities to exert immunoregulatory effects, thereby maintaining homeostasis of the organism.

CD8+ cytotoxic T cells

CD8+ cytotoxic T cells are considered the primary effector cells of anti-tumor immunity. Their restricted and antigen-specific recognition of tumor-associated antigens (TA) via class I MHC molecules, combined with their ability to produce perforin and other cytotoxins upon activation, enables them to induce tumor cell death while sparing normal cells.

Mouse and rat PBMC

In mice and rats, PBMCs are a crucial sample for studying immune responses due to their ease of collection and ability to reflect the host’s immune status. PBMCs from both species serve as a core tool in immunological research, with broad applications in elucidating disease mechanisms, drug development, and fundamental immunological studies. Standardized isolation and analytical protocols enable the efficient acquisition of highly active cells, providing reliable data for experimental investigations. Compared with tissue samples, mouse and rat PBMCs, being obtained non-invasively, preserve multiple immune cell subsets, making them well suited for multiparameter analysis.

In mice and rats, PBMCs are a crucial sample for studying immune responses due to their ease of collection and ability to reflect the host’s immune status. PBMCs from both species serve as a central tool in immunological research, with broad applications in elucidating disease mechanisms, drug development, and fundamental immunological studies. Standardized isolation and analytical protocols enable the efficient acquisition of highly active cells, thereby providing reliable data for experimental investigations. Compare with tissue samples, Peripheral blood mononuclear cells (PBMCs) from mice and rats can be obtained non-invasively, preserving multiple immune cell subsets and making them suitable for multiparameter analysis.

Main Applications of Mouse and Rat PBMCs

(1) Immunological Research

- Immune cell functional analysis: assessment of T/B cell proliferation and cytokine secretion (e.g., ELISPOT, flow cytometry).

- Infectious Disease and Vaccine Research: Evaluating immune responses induced by viruses (such as HIV and influenza) or vaccines.

(2) Autoimmune disease models

- Investigate abnormal activation or tolerance defects of PBMCs in diseases such as rheumatoid arthritis and multiple sclerosis.

(3) Tumor Immunotherapy

- Evaluate the effects of therapies such as CAR-T cells and PD-1 inhibitors on immune cells in PBMCs.

(4) Toxicology and Pharmacodynamics

- Testing the cytotoxicity or modulatory effects of drugs or compounds on immune cells (e.g., screening for immunosuppressants).

(5) Transplant Immunology

- Analyze the interactions between donor and recipient PBMCs in transplant rejection or graft-versus-host disease (GVHD).

(6) Metabolism and Aging Research

- Investigate changes in inflammatory markers (such as IL-6 and TNF-α) in PBMCs during obesity, diabetes, or aging.

Sample Number	Delivery Specifications	Delivery Format
HYS-SDP001C	Cryopreserve rat PBMCs, 1 million	Cryopreservation, single donor
HYS-SDP005C	Cryopreserve 5 million rat PBMCs.	Cryopreservation, single donor
HYS-SDP010C	Cryopreserve rat PBMCs, 10 million	Cryopreservation, single donor
HYS-SDP050C	Cryopreserve rat PBMC, 50 million	Cryopreservation, single donor
HYS-MSDP001C	Cryopreserve rat PBMCs, 1 million	Cryopreservation, mixed donor
HYS-MSDP005C	Cryopreserve 5 million rat PBMCs.	Cryopreservation, mixed donor
HYS-MSDP010C	Cryopreserve rat PBMCs, 10 million	Cryopreservation, mixed donor
HYS-MSDP050C	Cryopreserve rat PBMC, 50 million	Cryopreservation, mixed donor

Sample Number	Delivery Specifications	Delivery Format
HYS-MOSP001C	Cryopreserve mouse PBMCs, 1 million	Cryopreservation, single donor
HYS-MMOSP001C	Cryopreserve mouse PBMCs, 1 million	Cryopreservation, mixed donor
HYS-MMOSP005C	Cryopreserve mouse PBMCs, 5 million	Cryopreservation, single donor
HYS-MMOSP010C	Cryopreserve mouse PBMCs, 10 million	Cryopreservation, single donor
HYS-MMOSP020C	Cryopreserve mouse PBMCs, 20 million	Cryopreservation, mixed donor
HYS-MMOSP050C	Cryopreserve mouse PBMCs, 50 million	Cryopreservation, mixed donor
HYS-MOS010C-C57	Frozen mouse serum, 10 mL	Mixed-sex donor
HYS-MOSPB005C-C57	Cryopreserve 5 million mouse splenic B cells.