Hycells New Product | 4-Day Rapid DC Cell Induction Kit
Release Date:
2024-06-26 14:19
Introduction to Dendritic Cells
Dendritic cells (DCs) derive their name from the dendritic processes on their surface, which facilitate antigen capture and intercellular communication with other immune cells. As the most potent professional antigen-presenting cells (APCs), dendritic cells efficiently engulf, process, and present antigens. However, DCs are relatively rare in peripheral blood, accounting for only about 1% of peripheral blood mononuclear cells.
Hycells focuses on the broad clinical application prospects of dendritic cells in areas such as cancer immunotherapy, tumor vaccines, infectious disease treatment, autoimmune disease therapy, transplant immune tolerance, and immune monitoring and diagnostics. To this end, Hycells has developed a rapid dendritic cell induction kit and introduced an alternative method for monocyte sorting using magnetic beads—namely, the Attachment Solution—thereby establishing PBMC Direct DC induction protocol. Hycells Biotechnology can provide comprehensive induction. mDC For the iDC protocol and the 4-day rapid induction protocol for mDCs, detailed experimental guidelines can be obtained by contacting our company.
Product Information for the DC Induction Differentiation Kit
| Number | Product Name | Specifications |
| DCT01001-kit | Dendritic Cell Differentiation Kit (Monocyte-Derived, 50 mL) | 1Kit |
| DCT01002-kit | Dendritic Cell Differentiation Kit (Monocyte-Derived, 100 mL) | 1Kit |
| DCT01003-kit | Dendritic Cell Differentiation Kit (Monocyte-Derived, 200 mL) | 1Kit |
| DCT01001-A | Adhesion-promoting reagent | 50 μL |
| DCT01002-A | Adhesion-promoting reagent | 100 μL |
| DCT01003-A | Adhesion-promoting reagent | 200 μL |
No.1
Product Advantages of the Dendritic Cell Differentiation Kit
A 4-day induction protocol leads to rapid maturation and greater efficiency.
The 7-day induction regimen has higher purity.
The percentage of target cells is high, with a cell yield of 40%.
No.2
Product Advantages of Adhesion-Promoting Reagents
The replaceable monocyte sorting magnetic bead method and the direct induction of DC cells from PBMC cultures offer convenient operation and excellent cost-effectiveness!
Compared with the conventional adherent culture method, this approach significantly reduces the impact of donor-to-donor variability, enabling efficient induction across different batches of PBMCs.
Enhance sorting purity and induction yield.
Human DC cell differentiation culture system
| Training system | Culture medium volume | Cell inoculation amount |
| 24-well plate | 500ul | 5E5 |
| 6-well plate | 2mL | 2E6 |
| 10 cm dish | 10 milliliter | 1E7 |
Data Presentation for the DC Induction Differentiation Kit
1. Induce using this scheme Immature dendritic cells Flow cytometry results showed that, after 6 days of induction, approximately over 90% of the final cells were immature dendritic cells.
2. Induce using this scheme Mature dendritic cells Flow cytometry results showed that after 4 days of induction, more than 90% of the final cells were mature dendritic cells.
3. Induce using this scheme Mature dendritic cells Flow cytometry results showed that after 7 days of induction, the final cell population contained over 90% mature dendritic cells.
Microscopic Observation of DCs Induced from Cryopreserved PBMCs from Different Batches – Day 6
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