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Hycells Bio & InnoCell | Integrated Cell Subtype Sorting Solution

Release Date:

2025-08-21 15:35

Peripheral blood mononuclear cells (PBMCs) are a population of cells in peripheral blood that possess a single nucleus, primarily comprising lymphocytes and monocytes. According to the Biosecurity Law of the People’s Republic of China, which came into effect on April 15, 2021, human-derived samples must be obtained in a lawful and compliant manner. The Innosense Human Immune Cell Sorting Kit provides safe and reliable healthy donor-derived samples for use as controls.

 

Human-derived Immune Cell Sorting and Culture Kit

 

Leveraging a global pool of healthy donor resources and a comprehensive, end-to-end sample management system, Hycells is able to provide cell therapy companies, research institutions, and pharmaceutical firms with compliant, traceable, one-stop, high-quality biological sample solutions.

 

InnoCell Biotechnology is a biotechnology company focused on immune cell sorting, culture, and genetic regulation. The company has developed a comprehensive suite of reagent kits for immune cell sorting, including kits for human and mouse T cells and their various subsets, B cells, NK cells, monocytes, neutrophils, and other cell types.

 

Hycells Biotechnology has partnered with InnoSe to offer a high-quality sample resource base, combined with InnoSe’s specialized cell-sorting kits. These kits are easy to use, require minimal hands-on time, and deliver outstanding results in terms of cell purity, yield, and viability. Together, the two companies provide customers with an end-to-end, one-stop solution spanning the entire workflow—from sample acquisition to cell sorting—thereby accelerating their R&D and commercialization efforts.

 

Detailed product specifications for the HeYou Human-Derived Immune Cell + InnoCell Sorting and Culture Kit:

 

Item number Product Name

TZ3101(CD3)   

CD3 Sorting Kit Set

TZ3102(CD4)

CD4 Sorting Kit Set

TZ3103(CD8)

CD8 Sorting Kit Set

TZ3104 (Naive CD4)

Naive CD4 Sorting Kit Set

 

Product Composition: 

 

1: 10 million (1E+7) cryopreserved healthy PBMC samples

2: 1E+8 S Specification Sorting Kit (Human)

3: 500 mL Cell Sorting Buffer (FACS Buffer)

4: 100 mL human T lymphocyte culture medium (with serum)

5: 200 μL ActBeads™ Human T Cell Activation Magnetic Beads (Small Package)

 

 

 

 

Set promotion price: 1,999 yuan

 

 

 

 

Advantages:

1. The physical examination report, ethical approval documents, and other relevant materials are complete and compliant, thereby meeting the requirements for drug registration and sample traceability verification.

2. PBMC quality control is stringent, with at least eight routine flow cytometry-based quality-control assays performed to ensure that post-thaw cell viability, cell count, and cellular function remain consistent with pre-thaw levels.

3. Routine pharmacological efficacy verification: each batch of samples has undergone functional validation and screening.

 

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“TDCC assays typically refer to T cell–dependent cytotoxicity assays, which are a critical experimental approach for evaluating the tumor-killing efficacy of immunotherapeutic agents, such as bispecific T-cell engagers (TCEs).”

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Antibody-dependent cellular cytotoxicity (ADCC) is one of the key mechanisms of action for therapeutic monoclonal antibodies. At its core, effector cells expressing Fc receptors (FcγRs) kill target cells that express specific antigens by recognizing and binding to the antibody—specifically, the Fab region binds to the target-cell antigen while the Fc region remains exposed.

 

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“After sorting, activated T cells increase in size, and flow cytometric analysis clearly distinguishes between activated T cells and non-activated cells using the T-cell activation magnetic beads (AH2001). Within 24–48 hours of activation, surface markers such as CD25, CD69, ICOS, and CD71 are markedly upregulated. One method for assessing T-cell proliferation is dye-based cell-tracking, such as the CFSE proliferation assay. During T-cell proliferation, the dye is evenly distributed among daughter cells, resulting in a decrease in fluorescence intensity. The InnoScience cell-sorting kit combined with activation magnetic beads can effectively evaluate differences in T-cell proliferative capacity and is applicable to drug screening, gene-function studies, and other areas.”

 

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