Cell Feeder bottom-permeable cell culture flasks | High-yield protocol for immune cell expansion, no medium change or replenishment required after 7 days.
Release Date:
2025-08-11 15:34
Cell culture flasks serve as vessels for cell culture and expansion, offering the advantage of accommodating large cell densities and supporting long-term cultivation. These flasks typically feature a high surface-area-to-volume ratio, which promotes adherent cell growth and efficient nutrient exchange, facilitates subculturing and expansion, and meets the demands of research requiring large cell numbers. However, when using conventional cell culture flasks for cell expansion, frequent manual interventions are necessary to adjust cell density and replenish fresh culture medium, thereby increasing the risk of contamination. In contrast, permeable culture bags or bioreactors represent alternative approaches for large-scale production of CIK, T, and NK cells. That said, culture bags require substantial volumes of fresh medium and frequent adjustments of cell density, while monitoring cell status is inconvenient and relies on sampling or external sensors; bioreactors, on the other hand, are bulky, expensive, and complex to operate. The G-Rex series of culture flasks has been widely validated and adopted by users; nevertheless, this series is costly and subject to supply instability.
Hycells Biotechnology has meticulously engineered the Cell Feeder cell culture flask, a solution custom-designed for clients in the cell therapy field. This innovative product is aimed at significantly reducing hands-on operational time, effectively mitigating contamination risks, and addressing the challenge of batch-to-batch consistency in large-scale manufacturing. As an advanced integration that upgrades and merges traditional cell flasks with cell bags, the Cell Feeder offers customers a revolutionary new alternative. It not only delivers unprecedented convenience for CGT industry clients but also ensures the reliability and cost-effectiveness of cell production. Specifically tailored to build highly efficient manufacturing platforms, the Cell Feeder is particularly well-suited for the expansion of hematopoietic stem cells and immune cells—including T cells and NK cells—thereby propelling cell manufacturing to new heights.
Hycells delivers end-to-end optimization and a three-pronged, integrated approach, proudly unveiling a benchmark one-stop NK cell solution! NK seed cells + HiXpan Registered trademark The NK serum-free culture kit (cytokine-based), combined with the Cell Feeder bottom-permeable cell culture flask integrated culture system, provides end-to-end support for efficient, stable, and compliant NK cell expansion. After 7 days, no medium changes or supplements are required, enabling effortless acquisition of high-quality, high-density NK cells!
HiXpan Registered trademark NK Serum-Free Culture Kit (Cytokine-Based) & Cell Feeder Bottom-Permeable Cell Culture Flask: The Perfect Pair
Advantages of Cell Feeder Bottom-Permeable Cell Culture Flasks
Start Low initial cell culture yield: Culture can be initiated with as few as 50 million cells, eliminating the need for density-based media supplementation.
Custom-made : Specifically designed for the cell therapy field to meet specialized needs.
Unique breathable membrane design : The use of a breathable bottom membrane facilitates gas exchange and enhances cell culture efficiency.
Large-capacity medium filling : A large volume of culture medium can be added at once, eliminating the need for medium changes throughout the entire process and reducing the frequency of operational steps.
Efficient and convenient : Optimize operational processes, reduce labor costs, and improve production efficiency.
|
Name |
CELL FEEDER Bottom-Permeable Cell Culture Flask |
|
Dimensions (mm) |
280*125*100 |
|
Capacity |
1000 mL |
Advantages of Combined Use
High purity
HiXpan Registered trademark The NK cell serum-free culture kit (cytokine-based method) enables the generation of NK cell preparations with higher purity, thereby providing a stable foundation for clinical translation.
Efficient amplification
HiXpan Registered trademark The NK cell serum-free culture kit (cytokine-based) provides NK cells with abundant nutrients and growth factors, promoting rapid cell expansion.
Optimize transduction efficiency
HiXpan Registered trademark The NK serum-free culture kit (cytokine-based) can enhance the transduction efficiency of NK cells, laying the foundation for subsequent clinical applications.
Optimal Culturing Environment
The Cell Feeder bottom-permeable cell culture flask provides NK cells with an optimal gas exchange environment, which promotes cell growth and functional maintenance.
Verification Data Display
Using the Hycells NK serum-free culture system (cytokine-based method), PBMCs from batch 059K025 were cryopreserved at an initial cell count of 100 million. On Day 7, aliquots of 5 × 10^7, 1 × 10^8, and 2 × 10^8 cells were seeded into Cell Feeder cell culture flasks, with the medium volume directly supplemented to 1 liter. The remaining 1 × 10^8 cells were cultured in bioreactor bags, with density-adjusted medium replenishment performed as usual.
In summary:
Higher purity: Compared with cell culture bags, using Cell Feeder bottom-permeable cell culture flasks for NK cell cultivation results in approximately a 15% increase in purity, reduced interference from impurities, and more stable product quality.
Higher amplification efficiency Compared with traditional culture bags, NK cell expansion fold has increased by 50%, yielding a greater number of highly active cells and significantly reducing the per-cell culture cost.
Lower cost: Bottom-permeable cell culture flasks yield higher NK cell purity and offer significantly greater cost-effectiveness over the long term compared with conventional culture bags; they achieve a 50% increase in expansion fold, reduce media costs by 50%, and lower the per-cell cultivation cost.
Under identical conditions, cell feeder-based bottom-permeable cell culture flasks yield better expansion results when seeded at a lower density.
Steps for Using Cell Feeder Bottom-Permeable Cell Culture Flasks
Cell Feeder bottom-breathable cell culture flasks are suitable for the expansion and cultivation of various non-adherent cells. This product employs a dual-top-and-bottom breathable culture technology: during the culture process, cells settle on the bottom breathable membrane, allowing them to efficiently absorb nutrients from the culture medium while also receiving ample oxygen through the membrane, thereby promoting rapid cell proliferation.
Usage steps:
1. The initial activation and expansion are conducted exactly as in bag culture and must be performed in T-flasks.
2. After the cells have been thoroughly resuspended in the culture flask, first add 100 mL of culture medium and gently shake the flask to ensure uniform distribution of the cells across the bottom; then immediately add 900 mL of culture medium (including all supplements required during the culture process).
3. Transfer the bottom-permeable bottles into the incubator and allow them to incubate undisturbed for 7 days; after 7 days, remove them and harvest the culture.
4. During harvest, it is recommended to use a pipette or peristaltic pump to remove 80–90% of the culture medium. The pipette tip should be moved slowly from top to bottom to the desired cell layer height, avoiding direct insertion into the bottom to prevent excessive suction that could dislodge cell clumps. After removing 80–90% of the medium, gently shake the culture vessel to resuspend the remaining cells and medium, then tilt the vessel slightly and use the pipette to aspirate the remaining cells and medium.
Caution: When aspirating, do not allow the pipette tip to come into direct contact with the bottom membrane of the transparent bottle.
Cell Feeder Bottom-Permeable Cell Culture Flask Q&A
Q1: Which cell types are suitable for use with gas-permeable culture flasks?
Answer: Any suspension cells. Commonly used for CTLs, CAR-T cells, NK cells, TILs, Tregs, CIK cells, hybridoma cells, HeLa cells, SF9 cells, and others.
Q2: What are the advantages of using breathable culture flasks?
Answer:
1) Save labor time and costs;
2) The procedure is simple and convenient; during cultivation, there is no need for daily media replenishment, perfusion, shaking, or mixing, thereby reducing the risk of contamination.
3) High medium utilization efficiency, with higher proliferation rates and yields compared to culture bags of the same volume;
4) Enables high-density cultivation;
5) Requires minimal floor space, can be stacked for efficient use of incubator space.
Q3: Is there a standard cell culture protocol for each cell type, or is customization required?
Answer: Our protocol is a standard cell culture procedure that is effective for any suspension cell line. You can use it as a starting point and then optimize the seeding density as needed to shorten the culture cycle, minimize perturbations, and maximize expansion.
Q4: Is the training cycle fixed at 7 days?
Answer: Experimental results indicate that when harvesting cells after 7 days, the culture medium color typically shows little change. Depending on your specific cell-culture protocol and the desired cell yield, you can extend the culture period to further increase cell production.
Q5: Approximately what total cell count can be achieved when culturing cells in a cell-permeable culture flask?
Answer: The cell count is influenced by factors such as the cell type being cultured, the composition of the culture medium, the duration of cultivation, and the initial cell seeding density, among others; results vary depending on these conditions. Typically, a cell density of 1 million to 10 million cells per milliliter is observed.
Q6: Is cell counting required during the culture process?
Answer: We do not recommend performing daily cell counts. Data indicate that frequent counting during the culture process can alter the intracellular microenvironment, resulting in a 10–20% reduction in yield. Additionally, as cells tend to settle at the bottom of the culture vessel during incubation, sampling either the supernatant or the cell pellet at the bottom can lead to inaccurate technical data.
Q7: Can culture flasks be reused?
Answer: No, bottom-breath culture flasks are not for re-sterilization and must be used only once.
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