New Product LAB|HiXpan® NK Serum-Free Culture Kit (Cytokine-Based) for Stimulating CBNK Cell Cultures
Release Date:
2025-04-24 15:11
HiXpan Registered trademark NK Cell Serum-Free Culture Kit (Cytokine-Based) Validation data for CBNK stimulated culture in the 2L system
Stimulate the culture of CBNK
Time: 20250304–20250326
Sample 1: Cryopreserved CBMC, Batch No. CBMC DONOR1-20231108
Cell inoculation quantity: 21 million (3 × 10^6 cells/mL, 7 mL)
Initial NK cell purity: CD3−CD56+ proportion approximately 11.4%
01
Stimulus culture results
D0: 21 million CBMCs were seeded and cultured with stimulation for 20 days:
Approximately 8.85 billion umbilical cord blood NK cells were harvested;
Total cell expansion of approximately 421-fold;
The NK cell viability was 93.6%;
NK cell purity: CD3−CD56+ proportion approximately 95.0%, CD56+CD16+ proportion approximately 88.7%.
02
Flow cytometry result plot
Stimulate the culture of CBNK
Time: 20250304–20250326
Sample 2: Cryopreserved CBMC, Batch No. CBMC-20240312
Cell inoculum: 24 million (3 × 10^6 cells/mL, 8 mL)
Initial NK cell purity: CD3−CD56+ proportion approximately 10.3%.
01
Stimulus culture results
D0: 24 million CBMCs were seeded and cultured with stimulation for 20 days:
Approximately 12.3 billion umbilical cord blood NK cells were harvested;
Total cell expansion of approximately 512-fold;
The NK cell viability was 97.1%.
NK cell purity: CD3−CD56+ proportion approximately 84.7%, and CD56+CD16+ proportion approximately 84.1%.
02
Flow cytometry result plot
Stimulate the culture of CBNK
Date: 20250209–20250302
Sample 3: Cryopreserved CBMC, Batch No. JCBMC-20231016
Cell inoculum: 27.8 million (3 × 10^6 cells/mL, 9.27 mL)
Initial NK cell purity: CD3−CD56+ proportion approximately 11.7%
01
Stimulus culture results
D0: 27.8 million CBMCs were seeded and cultured with stimulation for 21 days:
Approximately 31.8 billion umbilical cord blood NK cells were harvested;
Total cell expansion of approximately 1,145-fold;
NK cell viability was 96.6%;
NK cell purity: CD3−CD56+ proportion approximately 98.3%, CD56+CD16+ proportion approximately 92.2%.
02
Flow cytometry result plot
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