Hycells New Product | Tackling the Challenges of Cryopreserving “Off-the-Shelf” NK and γδ T Cells
Release Date:
2024-04-12 14:01
Product Background Overview
Cell cryopreservation media serve as the liquid environment during cryopreservation, providing nutrients and protective effects to cells. They lower the freezing point, increase the permeability of cell membranes to water, and facilitate the efflux of intracellular water prior to freezing, thereby preventing or minimizing ice-crystal-induced damage. This allows cells to be temporarily suspended from active growth while preserving their cellular characteristics, so that they can be directly revived when needed to regain viability. Traditionally, cell cryopreservation media are prepared by mixing culture medium, serum, and DMSO in specific ratios; however, due to the complex composition of serum and significant batch-to-batch variability, their application is limited, and they require a stepwise cooling protocol for cryopreservation, which is time-consuming and labor-intensive.
Hycells’s newly launched serum-free cell cryopreservation solution is primarily intended for NK cells and γδ T cells T cells, dendritic cells (DCs), peripheral blood mononuclear cells (PBMCs), embryonic stem (ES) and induced pluripotent stem (iPS) cells, mesenchymal stem cells (MSCs), and other cell types have been validated to exhibit superior performance compared with comparable imported products.
1. Serum-free and free of animal-derived components.
2. Store at 2–8°C; no need for repeated freeze-thaw cycles.
[Product Principle]
Cell cryopreservation solutions contain a variety of cryoprotectants: some readily penetrate cells to prevent intracellular water from forming ice crystals that could damage the cells, while others cannot penetrate cells but can preferentially bind extracellular water molecules before ice crystal formation, thereby reducing the electrolyte concentration in the extracellular solution and limiting the influx of cations into the cell. Our company’s serum-free cell cryopreservation solution is formulated as a combination of multiple cryoprotectants, providing dual protection both inside and outside the cell and significantly enhancing cell recovery viability. The composition of our cell cryopreservation solution is well-defined, making it safer and more effective.
【Product Advantages】
Safety: Serum-free, xenogeneic component-free;
Precise positioning: It is suitable for the storage of immune cells and stem cells, with particularly excellent cryopreservation results for NK cells and γδ T cells.
Efficiency: High cell recovery rates, with recovery viability exceeding 90% for multiple cell types;
Convenience: Ready-to-use; no additional preparation required.
Simplicity: Suitable for cryopreservation of immune cells and stem cells, as well as long-term storage in liquid nitrogen at −196°C; simplified protocols for additional cell line types require validation prior to use.
Product Usage Instructions
1. Preparations Before Cell Cryopreservation
1) The viability of cryopreserved cells must be greater than 90% (the buffy coat fraction obtained from peripheral blood can be cryopreserved immediately).
2) Calculate cell density; refer to the attached table for recommended cryopreservation densities for different cell types.
2. Cell Cryopreservation Process
1) Count the cell suspension to be cryopreserved, then centrifuge at 400 × g for 10 minutes.
2) Discard the supernatant, then slowly add the 4°C–stored cell cryopreservation solution to the pelleted cells, adjusting the volume of the cryopreservation solution based on cell density.
3) After repeated pipetting and mixing, aliquot the solution into cryovials, 500–1000 µL per vial.
4) Place the aliquoted cell cryovials in a programmed cooling box or a programmed freezer, then transfer them to liquid nitrogen for long-term storage.
Note: Cell cryovials must be completely sealed; otherwise, they may rupture during the thawing process.
3. Cell Revival Process
1) Turn on the water bath in advance and set the temperature to 37°C.
2) Remove the cryovial from liquid nitrogen and promptly place it in a water bath to thaw, aiming to complete thawing within 1 minute; the shorter the thawing time, the lesser the impact on the cells.
3) Quickly dispense the melted cell-containing cryopreservation solution into fresh culture medium and mix thoroughly. For example, if the cryopreservation solution volume is 500 µL, add it to 5 mL of complete culture medium; if the cryopreservation solution volume is 1 mL, add it to 10 mL of complete culture medium.
4) After thorough mixing, centrifuge immediately at 400 × g for 10 minutes. Upon completion of centrifugation, discard the supernatant and add pre-warmed fresh culture medium.
5) Slowly add an appropriate volume of fresh culture medium to the cell pellet, gently mix to ensure uniform suspension, and then transfer the well-mixed cells into pre-prepared culture vessels. After mixing, aliquot the cells into flasks and incubate in a CO2 incubator (with the CO2 concentration determined according to the requirements of the culture medium, typically 5%).
Appendix:
Precautions:
1. During cryopreservation, after adding the cryoprotective solution to the cells, perform all procedures as close as possible to an ice pack, as low temperatures help prevent damage to the cells caused by the cryoprotectant.
2. Ensure that cryovials are completely sealed to prevent rupture during the thawing process.
3. This product is supplied in sterile packaging and does not require filtration; please ensure aseptic handling during use.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
Presentation of Application Data for Serum-Free Cell Cryopreservation Solution
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Phone: 021-36696819
Mobile phone:13061948224(Ms. Yu)
Email:monika@hys-bio.com
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