ChemoMetec Fully Automated Cell Counter | More Accurate Counts, Higher Standards!
Release Date:
2023-03-22 13:09
Part 01
Principle and Introduction to Cell Counters
The cell therapy and gene therapy industries are experiencing rapid growth, and to ensure the quality of their final products, precise cell counting is essential at every stage and in every process. From Denmark, ChemoMetec is a global leader in the manufacture of cell counting and analysis equipment. , focuses on the development and manufacture of high-precision instruments, providing accurate data to thousands of customers in the biotechnology, pharmaceutical, and academic sectors. Its products NucleoCounter® Series of Cell Counters NC-200™, NC-250™, and NC-3000™ ， Use Advanced cell image analysis and fluorescence staining techniques for determining cell count and viability. , providing the highest level of assurance for the precision, stability, and consistency of cell-counting processes. The company is currently successfully listed on the NASDAQ Stock Market.
NucleoCounter® Series Cell Counters
The NucleoCounter®, by utilizing the single-use consumable Via1-Cassette™, will The three steps of sample loading, staining, and counting are combined into one. , which simply yet effectively enhances the accuracy of counting results. The core of the NucleoCounter® cell counter lies in its unique counting methodology. The instrument employs the Via1-Cassette™, which uses a nuclear fluorescent dye to automatically stain cell nuclei, enabling the counter to accurately detect cells in various states: live cells, dead cells, cell clusters, and low-viability cells. In addition, Software that complies with Good Manufacturing Practice (GMP) and is easy to operate , which is another highlight of the NucleoCounter® cell counter.
The cell counting chamber features side-opening design at the bottom, eliminating concerns about sample blockage. A built-in waste collection reservoir ensures that the cell suspension is directly discharged into the reservoir, thereby preventing any risk of biological contamination. In addition, each cell counting chamber is equipped with identification markers for recording factory-specific information; during counting, the instrument automatically detects the chamber’s thickness and the volume of sample in the counting area, thus minimizing measurement errors inherent in industrial production.
The flagship product of the NucleoCounter® series of cell counters, the NC-200™, is Using a fixed focal length Perform photo-based sampling to minimize wear and error caused by manual operation and autofocus.
The AO/DAPI dye built into the NucleoCounter® NC-200™ does not cause changes in cell viability over time, unlike trypan blue staining. It also reduces safety risks for laboratory personnel. Compared with AO/PI, the excitation spectra of AO and DAPI overlap very little and do not interfere with each other, allowing detection of the region with the strongest excitation signal. In contrast, AO and PI have highly overlapping excitation spectra and can mutually quench each other’s fluorescence, necessitating the use of very narrow-band filters to isolate the signal, which results in only weak signals being detected from the marginal regions.
At the same time, the NucleoCounter® NC-200™ can analyze a wide range of cell samples, offering flexible compatibility with mammalian cells, whole blood cells, epithelial cells, and adipocytes. Moreover, the NucleoCounter® NC-200™ provides dedicated protocols for handling cells cultured in 3D systems as well as those grown on microcarriers.
Part 02
Summary of Product Advantages
Precise cell detection
NucleoCounting analyzes samples by detecting two nuclear fluorescent dyes—Acridine Orange and DAPI—that are pre-loaded into the Via1-Cassette™. Acridine Orange stains all cells in the sample green, while DAPI selectively stains only dead cells blue.
Through meticulous image analysis and computation, fluorescent images of cells are acquired and analyzed, with their fluorescence intensity and size recorded.
By analyzing only cell nuclei rather than intact cells, the method avoids the need to examine particles that do not contain DNA information. The NucleoCounter® enables precise detection of every cell in the sample, including cell aggregates and cell debris, thereby further enhancing the reliability of the counting results.
Precise cell counting
The Via1-Cassette™ is our core technology for achieving high-precision cell counting, integrating sample loading, staining, and the counting chamber into a single unit. Each Via1-Cassette™ is marked with a dot code that indicates the volume of its counting chamber; the NucleoCounter® counter automatically reads this information when calculating cell concentration. The NucleoCounter® employs a fixed-focus lens to capture images, eliminating the need for manual or automatic focusing during counting. By combining the Via1-Cassette™ with the NucleoCounter®, numerous sources of variability are eliminated at the source, thereby enhancing the overall accuracy and reliability of cell counting. Supported sample types include primary cells, low-viability cells, whole blood cells, adipose-derived mesenchymal stem cells, microcarriers, cell–magnetic bead mixtures, differentiated embryonic stem cells, and 3D-cultured cells, among others.
Streamlined Cell Counting Pipeline
The NucleoCounter® cell counter is designed for use in manufacturing environments. Its straightforward two-step workflow significantly reduces human error in cell counting, ensuring consistent measurement results across different users. The Via1-Cassette™ and the NucleoCounter® eliminate several steps inherent in traditional cell-counting methods, thereby minimizing variability introduced by operator differences.
Simple Device Verification
The NucleoView software includes built-in validation procedures that facilitate Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ). In principle, the NucleoCounter® counter requires no calibration or routine maintenance. Regular use of the IQ/OQ kit enables verification of instrument stability.
Easily monitor through reporting and approval tools.
NucleoView® software automatically generates detailed PDF reports, enabling users to monitor and approve cell count and viability results. The reports also include data on cell size and aggregate formation, as well as charts that highlight sample bias.
Stability
All NucleoCounter® cell counters deliver equally accurate counts, regardless of manufacturing location or date of shipment. Following final assembly, each instrument undergoes testing and quality control after being calibrated against a master reference instrument. The NucleoCounter® cell counter requires no routine maintenance or recalibration; periodic laboratory method validation can be achieved using OQ and PQ kits.
21 CFR Part 11
NucleoCounter® NC-200™ data can be stored locally or on a network. Another key feature of the NucleoView™ software is its inclusion of a module compliant with 21 CFR Part 11, enabling electronic recordkeeping, electronic signatures, and the management of paperless record-keeping systems in full compliance with cGMP regulations. Consequently, the NucleoCounter® is ideally suited for manufacturing environments.
Widely used on the GMP platform
Most customers use the NucleoCounter® cell counter to support cutting-edge scientific research applications, such as cell therapy, bioprocess manufacturing, vaccine production, and cell biology studies. The extensive and rigorous evaluations conducted by these companies fully demonstrate the NucleoCounter®’s outstanding performance in OQ and GMP manufacturing processes. In the early stages of R&D, adopting the NucleoCounter® at the earliest possible opportunity helps ensure the consistency and objectivity of cell counting at every subsequent step.
The Ideal Choice for Cleanrooms
The NucleoCounter® cell counter is an ideal instrument for cleanroom environments. Its fanless design and smooth, easy-to-clean surface minimize the risk of contamination without compromising performance. In addition, ChemoMetec reagents, which are conveniently packaged, facilitate their transfer into cleanrooms.
Part 03
Hycells appointed as ChemoMetec agent
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