Small “Life” Makes Sense (8) | Pre-Screening of Reconstructed Humanized Hu-PBMC Mouse Strains
Release Date:
2024-10-21 16:39
As an advanced experimental animal model, humanized immune system mice have demonstrated tremendous potential and practical value in biomedical research and drug development in recent years. The generation of such mice typically relies on immunodeficient mouse models, in which human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), or other immune tissues are transplanted into these mice to reconstruct a human immune system, thereby mimicking human immune characteristics and pathological processes.
Among these, the PBMC-humanized mouse model (Hu-PBMC model) is generated by transplanting human peripheral blood mononuclear cells into immunodeficient mice, thereby endowing the mice with partial human immune functions. These cells encompass a variety of immune cell types, including T cells, B cells, and NK cells. This model plays an irreplaceable role in pharmacodynamic studies of immunotherapies and in cancer research. Despite the numerous advantages of the PBMC-humanized mouse model, PBMCs from different donors exhibit variability in their engraftment capacity, their interactions with tumor cells, and their responses to immunotherapeutic agents. To ensure the accuracy and reliability of experiments, rigorous pre-screening is required prior to formal experimentation. The primary objective of pre-screening is to identify PBMC donors that demonstrate stable engraftment and compatibility with tumor cells, thereby enhancing the success rate and reproducibility of the experiments.
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