New Product | Hycells Announces the Launch of High-Viability Primary Hepatocyte Cells!
Release Date:
2026-02-11 16:51
Primary hepatocytes
Primary hepatocytes They are functional cells directly isolated from liver tissue and serve as an ideal in vitro model for studying drug metabolism, drug–drug interactions, hepatotoxicity, and transporter activity, making them highly valuable in drug ADMET evaluation—particularly in drug metabolism and toxicology research. As highly differentiated parenchymal cells, they retain core physiological functions such as metabolism, detoxification, protein synthesis, and bile secretion, and offer the following advantages:
The enzyme system and cofactors are maintained at physiological levels, enabling the study of drug metabolism and toxicity under conditions that closely mimic the in vivo environment.
The cells maintain good morphology and metabolic activity, accurately reflecting in vivo metabolic characteristics.
Adhering to the “3Rs” ethical principles in animal research helps reduce ethical controversies and lower experimental costs.
Primary hepatocytes after isolation can be cultured in suspension or adherent conditions, making them suitable for various research applications.
01 Suspended Culture of Hepatocytes
Within 4 hours after recovery, its cytochrome P450 enzyme activity is most similar to that in vivo, making it suitable for short-term metabolic studies.
Product Features
Multiple donors are cryopreserved in a single batch, with each batch containing more than 100 tubes.
Revival rate ≥ 80%
Live cell count > 5 × 10 6 /pipe
Provide CYP enzyme metabolic activity data
02 Adherent Culture of Hepatocytes
It exhibits good recovery and structural stability, making it suitable for long-term, complex studies on the stability of cytotoxic, enzyme-inducing, and slowly metabolized drugs.
In addition, hepatocyte plasma membranes highly express the asialoglycoprotein receptor (ASGPR), which binds galactosyl-N-acetylgalactosamine (GalNAc) with high affinity and is frequently used in research on liver-targeted delivery of small nucleic acid therapeutics such as siRNA, making it suitable for uptake and transfection-related experiments.
Product Features
Multiple donors are cryopreserved in a single batch, with each batch containing more than 50 tubes.
Revival rate ≥ 80%
Adhesion verification ≥ 72 hours
Applications of Suspension and Adherent Culture
Applications of Suspension Culture
1. Short-term drug metabolism studies
This includes assessments of metabolic stability and identification of metabolites (particularly those involved in phase II metabolic reactions), making it suitable for experiments that require rapid characterization of in vivo metabolic profiles.
2. High-Throughput Toxicity Screening
Used for acute toxicity testing and initial hepatotoxicity screening of compound libraries, supporting large-scale drug screening.
3. Disease Model Construction
It can be used to establish in vitro models of viral hepatitis, non-alcoholic fatty liver disease, and other conditions for studying disease mechanisms.
Applications of Adherent Culture
1. Long-term Metabolic and Toxicity Studies
Suitable for enzyme induction studies, stability assessment of slow-metabolizing drugs, and analysis of accumulation effects.
2. Study of Cytotoxic Mechanisms
Supports the establishment of acute/chronic liver injury models for mechanistic studies.
3. Disease Models and Therapeutic Development
It can be used to establish models of hepatitis, liver cirrhosis, hepatocellular carcinoma, and other conditions, as well as for antiviral drug screening.
4. Research on Gene and Cell Therapy
Applicable to research related to gene editing, cell transplantation, and bioartificial liver support systems, among others.
Suspension Culture vs. Adherent Culture
Selection Recommendations
For short-term assays, such as metabolic stability and acute toxicity studies, suspension culture is preferred to save time and reduce costs.
Adherent culture is preferred for long-term functional studies (such as enzyme induction and chronic toxicity) or for modeling complex diseases, in order to maintain cellular polarity and functional integrity.
High-throughput screening can enhance efficiency by integrating suspension culture with automated platforms, such as liquid-handling workstations.
Preclinical studies, such as those on bioartificial livers, require adherent cell culture to more accurately mimic the in vivo microenvironment.
03 Core Advantages of Hycells Biotechnology
1. Clear sourcing, broad species coverage, and guaranteed quality
Hepatocytes from mice, rats, dogs, cats, pigs, and rabbits: Use compliant, healthy, young experimental animals to ensure high cell viability and consistency.
Cynomolgus monkey hepatocytes: traceable origins, compliant with animal welfare guidelines, and supportive of translational medicine research.
2. Standardized Preparation Process
High viability: Fresh hepatocyte viability ≥90%; cryopreserved hepatocyte viability ≥80%, with some batches exceeding 90% (all viability figures are reported without Percoll purification).
High Purity: Hepatocyte purity ≥95%, minimizing interference from non-parenchymal cells;
High adhesion rate: Optimized cryopreservation technology ensures a post-thaw cell viability of ≥85% and an adhesion rate exceeding 90%, supporting long-term, stable experiments.
Enzyme activity data: Phase I enzyme activity data, Phase II enzyme activity data.
Fast Delivery: End-to-end cold-chain management ensures nationwide delivery within 24–72 hours.
3. Ready-to-use adherent culture
Cells can adhere rapidly after pre-processing and are ready to use right out of the box;
Pre-paved plate culture kits are available as an option, along with dedicated culture media and cultivation recommendations.
Provide cell function validation data (key metrics such as confluence, CYP450 enzyme induction, and ICG uptake).
Data Presentation of Adherent Animal Hepatocytes
01 C57BL/6 mice
Cell morphology: Post plating (12W-plate)
0.2M-5H 0.2M-24H
02 CD-1 Mouse
Cell morphology: Post plating (12W-plate)
0.25M-6H 0.25M-24H
03 Crab-eating Macaque
Cell morphology: Post plating (12W-plate)
0.6M-6H 0.6M-24H
04 SD rats
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